Cat. #151773
HBRN Mouse
Cat. #: 151773
Sub-type: Mouse
Availability: 8-10 weeks
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Colin Henderson ; Roland Wolf
Institute: University of Dundee
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: HBRN Mouse
- Research fields: Cell signaling and signal transduction;Genetics;Metabolism
- Tool sub type: Mouse
- Conditional: No
- Description: Improved model of abrogation of hepatic P450 function. The dual deletion of both Cyb5 and POR almost completely abrogates all hepatic cytochrome P450 activities; the new HBRN model thus provides a more authentic hepatic-P450 null phenotype
- Genetic background: HBRN (Cytb5lox/lox::PORlox/lox CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.
- Zygosity: HBRN (Cytb5lox/lox::PORlox/lox ą CreALB)
- Production details: HBRN (Cytb5lox/lox::PORlox/lox Âą CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: P450, Cyb5
References
- Henderson et al. 2013. Mol Pharmacol. 83(6):1209-17. PMID: 23530090.
- Evidence that cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the hepatic cytochrome P450 system.
- Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.
- Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase.
