#151443

GSTP1GSTP2 KO Mouse

Cat. #151443

GSTP1GSTP2 KO Mouse

Cat. #: 151443

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

Model: Knock-Out

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Roland Wolf ; Colin Henderson

Institute: University of Dundee

Primary Citation: Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266

Tool Details
Handling
Target Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: GSTP1GSTP2 KO Mouse
  • Cancer: Lung cancer
  • Cancers detailed: Lung adenoma
  • Research fields: Cancer;Genetics
  • Tool sub type: Mouse
  • Disease: Cancer
  • Model: Knock-Out
  • Conditional: No
  • Description: In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)
  • Application: Lung and skin tumourigenesis modeling; drug metabolism; drug toxicity studies
  • Genetic background: A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout.
  • Phenotype: Tumourigenesis (lung, skin); drug metabolism; drug toxicity
  • Zygosity: Homozygous
  • Production details: A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Glutathione Transferase P1 and P2 (GSTP1, GSTP2)
  • Target background: Regulator of protein synthesis/degradation

Application Details

  • Application: Lung and skin tumourigenesis modeling; drug metabolism; drug toxicity studies

References

  • Zhou et al. 2008. Am J Respir Crit Care Med. 178(12):1202-10. PMID: 18787219
  • Elsby et al. 2003. J Biol Chem. 278(25):22243-9. PMID: 12646564
  • Rosario et al. 2000. Mol Pharmacol. 58(1):167-74. PMID: 10860939
  • Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266