GSTP1GSTP2 KO Mouse

In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)

Contributors

Inventor Institute
Roland Wolf ; Colin Henderson University of Dundee
Cat. #: 151443
Cancer types: Lung cancer
Research Fields: Cancer;Genetics
Target: Glutathione Transferase P1 and P2 (GSTP1, GSTP2)
disease: Cancer
Model: Knock-Out
Zygosity: Homozygous
Phenotype: Tumourigenesis (lung, skin); drug metabolism; drug toxicity
Primary citation: Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266
Product description: In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)
Conditional: No
Genetic background: A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout.
Production details: A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Zhou et al. 2008. Am J Respir Crit Care Med. 178(12):1202-10. PMID: 18787219

Elsby et al. 2003. J Biol Chem. 278(25):22243-9. PMID: 12646564

Rosario et al. 2000. Mol Pharmacol. 58(1):167-74. PMID: 10860939

Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266

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