Cat. #161938
SK-OV-3 Cell Line
Cat. #: 161938
Availability: 8-10 weeks
Tissue: Ovary; Ascites
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-OV-3 Cell Line
- Cancer: Gynaecologic cancer
- Cancers detailed: Adenocarcinoma
- Gender: Female
- Tissue: Ovary; Ascites
- Donor: This cell line was established in 1973 from the ascites of a 64-year-old Caucasian female with adenocarcinoma of the ovary.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del(1)(q21), der(13)t(1;?;13) (q11;?;q34), der(11)t(11;?) (q12), del(10)(q22) and 3 other marker chromosomes were common to most cells, and 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 and N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged and unassignable chromosomes. The X chromosome was either single or paired. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1-2 PGM3, 1
- Description: SK-OV-3 is a human ovarian cancer cell line with epithelial-like morphology.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type B; Rh+
Applications
- Application: 3D cell culture
Handling
- Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: XCSF1PO: 11D13S317: 8,11D16S539: 12D5S818: 11D7S820: 13,14TH01: 9,9.3TPOX: 8,11vWA: 17,18D3S1358: 14D21S11: 30,31.2D18S51: 16,17Penta_E: 5,13Penta_D: 12,13D8S1179: 14,15FGA: 23,24,25D19S433: 14,14.2D2S1338: 18,23
References
- Shaw et al. 2004. Molecular therapy : the journal of the American Society of Gene Therapy. 10(6):1032-1042. PMID: 15564135.
