#154858

SIRMu-1 Cell Line

Cat. #154858

SIRMu-1 Cell Line

Cat. #: 154858

Unit size: 1x10^6 cells / vial

Organism: Rat

Tissue: Eye

Disease: Retinal studies into Muller cell function

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Daniel Peet

Institute: The University of Adelaide

Tool Details
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: SIRMu-1 Cell Line
  • Research fields: Drug development;Neurobiology
  • Organism: Rat
  • Tissue: Eye
  • Disease: Retinal studies into Muller cell function
  • Growth properties: large and flat with a "ghost-like" appearance.
  • Model: Immortalised Line
  • Description: Both the cellular morphology and overall transcriptome of the SIRMu-1?cells are more similar to primary rat MCs than the commonly used rMC-1?cells
  • Production details: A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution. No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial...
  • Cellosaurus id: CVCL_UW38

Handling

  • Format: Frozen
  • Growth medium: MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle's Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice