To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of MDM2 inhibitors, Nutlin-3, and a clonal resistant cell line generated. The p53 mediated responses of parental and resistant cell lines were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell lines.
Conditional:
No
Production details:
Resistant cell lines were established by exposing SJSA-1 cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones.
Cellosaurus ID:
CVCL_HG10
Parental cell line:
SJSA-1
Disease:
Cancer
Cat. #:
152838
Tool sub type:
Continuous
Unit size:
1×10^6 cells / vial
Cancer types:
Sarcoma
Research Fields:
Apoptosis and autophagy;Cancer;Cell biology;Drug development;Genetics
Tissue:
Bone
Model:
Cancer Model
Growth properties:
Adherent
Cancer Types In Detail:
Multipotential sarcoma;Osteosarcoma
Format:
Frozen
Storage conditions:
Liquid Nitrogen
Shipping conditions:
Dry ice
Mycoplasma free:
Yes
Biosafety level:
1
References:
TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.
Drummond et al. 2016. Oncotarget. :. PMID: 27323823.
Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.
