S_N40R2 Cell Line

To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of MDM2 inhibitors, Nutlin-3, and a clonal resistant cell line generated. The p53 mediated responses of parental and resistant cell lines were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell lines.

Contributors

Inventor Institute
John Lunec Newcastle University
SKU: 152838
Product description: To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of MDM2 inhibitors, Nutlin-3, and a clonal resistant cell line generated. The p53 mediated responses of parental and resistant cell lines were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell lines.
Conditional: No
Production details: Resistant cell lines were established by exposing SJSA-1 cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones.
Cellosaurus ID: CVCL_HG10
Parental cell line: SJSA-1
Disease: Cancer
Cat. #: 152838
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Sarcoma
Research Fields: Apoptosis and autophagy;Cancer;Cell biology;Drug development;Genetics
Tissue: Bone
Model: Cancer Model
Growth properties: Adherent
Cancer Types In Detail: Multipotential sarcoma;Osteosarcoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Mycoplasma free: Yes
Biosafety level: 1
References:

TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.

Drummond et al. 2016. Oncotarget. :. PMID: 27323823.

Images

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S_N40R2 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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