#153463

Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6]

Please Enter Your Email ID
Email ID

Cat. #153463

Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6]

Cat. #: 153463

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Eye

Model: Reporter

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jonathon Pines

Institute: University of Cambridge

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6]
  • Alternate name: CCNA2
  • Research fields: Cell biology
  • Parental cell: RPE1
  • Organism: Human
  • Tissue: Eye
  • Model: Reporter
  • Conditional: No
  • Description: Somatic knock-in reporter cell line with endogenous expression of Cyclin A2 fused to the yellow fluorescent protein Venus (Cyclin A2-Venus fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A binds and activates CDC2 or CDK2 kinases, and thus this cell line functions as a reporter for both cell cycle G1/S and G2/M transitions. See also the Mad2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Cyclin A2 levels and activity.
  • Production details: To generate the cell line recombinant adenovirus-associated virus (rAAV)-mediated gene targeting was used to introduce the open reading frame (ORF) of yellow fluorescent protein (Venus) into the last exon of one allele of the CCNA2 (Cyclin A2) gene in hTert-RPE1 cells (RPE1; retinal pigment epithelial). RPE1 cells were chosen because they have a normal diploid karyotype, are not transformed and exhibit little cell death when arrested in mitosis; tagging the endogenous Cyclin A2 protein in RP...
  • Biosafety level: 1

Target Details

  • Target: Cyclin A2

Handling

  • Format: Frozen
  • Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?„?‚ƒ?‚¤(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] ; Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] ; Ruby-Mad2 Reporter Cell Line

References

  • Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242