#161898

OV-2978

Cat. #161898

OV-2978

Cat. #: 161898

Organism: Human

Tissue: Ovary

Disease: Cancer

£575.00

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Contributor

Inventor: Anne-Marie Mes-Masson and Diane Provencher

Institute: Centre Hospitalier de L’université de Montréal

Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: OV-2978
  • Organism: Human
  • Tissue: Ovary
  • Donor: Donor was 63 at diagnosis, BRCA hereditary status: negative, Grade 3 – Stage IIIC, Mutations: TP53, Chemonaive at time of sampling, and no prior history of cancer
  • Disease: Cancer
  • Morphology: Formed loose aggregates rather than solid spheroids
  • Growth properties: Mixed
  • Crispr: No
  • Description: Epithelial ovarian cancer cell line derived from ascites of patient diagnosed with Stage 3 IIIC ovarian high-grade serous adenocarcinoma who was chemotherapy naive at time of sampling. Exhibited strong sensitivity to carboplatin
  • Production details: Ascites were centrifuged and cellular fraction was seeded in 100mm place with OSE medium for 40 days with weekly culture medium replacement
  • Biosafety level: 1

Handling

  • Format: Frozen
  • Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
  • Temperature: 37° C
  • Atmosphere: 7% O2 and 5% CO2
  • Shipping conditions: Dry Ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
  • Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
  • Str profiling: TH01: 8 / D21S11: 30,33.2 / D5S818: 12 / D13S317: 12 / D7S820: 10,12 / D16S539: 12,13 / CSF1PO: 11,12 / vWA: 16,17 / TPOX: 8 / AMEL: X

References

  • Meng, et al. 2023. J Anim Sci. 3, 101:skad215. PMID: 37351870
  • Borzdzi?owska, et al. 2022. Biomedicines. 11, 10(5):1116. PMID: 35625852
  • Guo, et al. 2022. Front Genet. 25, 13:844800. PMID: 35401671
  • Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519