Cat. #161898
OV-2978
Cat. #: 161898
Organism: Human
Tissue: Ovary
Disease: Cancer
£575.00
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Contributor
Inventor: Anne-Marie Mes-Masson and Diane Provencher
Institute: Centre Hospitalier de L’université de Montréal
Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: OV-2978
- Cancer: Gynaecologic cancer
- Research fields: Cancer
- Organism: Human
- Tissue: Ovary
- Donor: Donor was 63 at diagnosis, BRCA hereditary status: negative, Grade 3 – Stage IIIC, Mutations: TP53, Chemonaive at time of sampling, and no prior history of cancer
- Disease: Cancer
- Morphology: Formed loose aggregates rather than solid spheroids
- Growth properties: Mixed
- Crispr: No
- Description: Epithelial ovarian cancer cell line derived from ascites of patient diagnosed with Stage 3 IIIC ovarian high-grade serous adenocarcinoma who was chemotherapy naive at time of sampling. Exhibited strong sensitivity to carboplatin
- Production details: Ascites were centrifuged and cellular fraction was seeded in 100mm place with OSE medium for 40 days with weekly culture medium replacement
- Biosafety level: 1
Handling
- Format: Frozen
- Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
- Temperature: 37° C
- Atmosphere: 7% O2 and 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
- Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
- Str profiling: TH01: 8 / D21S11: 30,33.2 / D5S818: 12 / D13S317: 12 / D7S820: 10,12 / D16S539: 12,13 / CSF1PO: 11,12 / vWA: 16,17 / TPOX: 8 / AMEL: X
References
- Meng, et al. 2023. J Anim Sci. 3, 101:skad215. PMID: 37351870
- Borzdzi?owska, et al. 2022. Biomedicines. 11, 10(5):1116. PMID: 35625852
- Guo, et al. 2022. Front Genet. 25, 13:844800. PMID: 35401671
- Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519
