#161890

OCM.66-1

Cat. #161890

OCM.66-1

Cat. #: 161890

Organism: Human

Tissue: Ovary. Ovarian/primary peritoneal. NOTE: The precursor of a substantial proportion of HGSOC is likely to be serous tubal intraepithelial carcinoma in the fimbriae of the fallopian tubes

£575.00

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Contributor

Inventor: Louisa Nelson and Stephen S. Taylor

Institute: University of Manchester

Primary Citation: Louisa Nelson et al. 2020. Nat Commun. 13,11(1):822. PMID: 32054838

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: OCM.66-1
  • Organism: Human
  • Gender: Female
  • Tissue: Ovary. Ovarian/primary peritoneal. NOTE: The precursor of a substantial proportion of HGSOC is likely to be serous tubal intraepithelial carcinoma in the fimbriae of the fallopian tubes
  • Donor: Age at diagnosis: 64; Previously treated (1 line; response = PD)
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model description: FIGO 3C; Ascites; TP53 mutation (p.Y163C)
  • Crispr: No
  • Description: The Taylor lab Ovarian Cancer Models (OCMs) are patient-derived tumour cell cultures, unfettered by contaminating, genetically normal stromal cells and the microenvironment. OCMs display the hallmark characteristics of HGSOC and retain the unique molecular features of the original tumour. OCMs have extensive proliferative potential, enabling high-resolution cell biology and drug-sensitivity profiling on tumour cells ‘close to the patient’, i.e. without extensive culture and genetic drift. OC...
  • Application: Ex vivo drug-sensitivity profiling; OCM-derived xenograft generation
  • Production details: To establish cultures, red blood cells were lysed, the remaining cellular fraction harvested by centrifugation, disaggregated if necessary then plated in OCMI media. Serial passaging and selective detachment eliminated white blood cells and yielded separate tumour and stromal fractions.
  • Biosafety level: 2

Applications

  • Application: Ex vivo drug-sensitivity profiling; OCM-derived xenograft generation

Handling

  • Format: Frozen
  • Passage number: Approx. P20
  • Growth medium: OCMI (PMID: 26080861; 32054838) + 5% hyclone FBS
  • Temperature: 37° C
  • Atmosphere: 5% CO2 & 5% O2
  • Shipping conditions: Dry ice
  • Storage medium: Bambanker freezing solution
  • Storage conditions: Liquid nitrogen (1/2 T75 ISO)
  • Initial handling information: Primaria™ Tissue Culture Flasks
  • Subculture routine: Split sub-confluent cultures (~90%) 1:2 using 0.25% trypsin. Limit trypsin incubation to 3 mins max, multiple applications of trypsin may be required to remove all the cells from the flask. Trypsin should be neutralised using DMEM containing 20% FBS
  • Cultured in antibiotics: Penicillin and Streptomycin
  • Mycoplasma free: Yes
  • Characterisation tests: TP53 mutation status confirmed by Sanger sequencing of RT-PCR products

References

  • Bethany M Barnes et al. 2021. Genome Med. 1,13(1):140. PMID: 34470661
  • Camilla Coulson-Gilmer et al. 2021. J Exp Clin Cancer Res. 16,40(1):323. PMID: 34656146
  • Anya Golder et al. 2022. NAR Cancer. 12,4(4):zcac036. PMID: 36381271
  • Louisa Nelson et al. 2020. Nat Commun. 13,11(1):822. PMID: 32054838