NUCOLL43 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: NUCOLL43 Cell Line
• Alternate name: O-CCC, ovarian clear cell carcinoma, HGSC, high grade ovarian cancer, TP53, CA125, LOH, loss of heterozygosity
• Cancer: Gynaecologic cancer
• Cancers detailed: Ovarian clear cell carcinoma
• Research fields: Cancer;Cell biology;Genetics
• Tool sub type: Primary
• Parental cell: Clear cell adenocarcinoma of gynecological origin
• Organism: Human
• Gender: Female
• Tissue: Ovary
• Disease: Cancer
• Growth properties: Doubling time approximately 45 hours
• Model: Cancer Model
• Conditional: No
• Description: Ovarian cancer is a significant cause of death in women worldwide with the majority of ovarian cancers forming in women under the age of 65. Due to late stage diagnosis and a lack of reliable screening tests, survival rates after 5 years are below 50% in developing counties. Ovarian cancer can spread to tissues in close proximity to the ovaries, including the lining of the abdomen, lungs, lymph nodes and liver. Ovarian cancer has a number of histological subtypes and ovarian clear cell carcinomas generally respond poorly to chemotherapy. NUCOLL43 was established from ascitic fluid from a female 57 year old white British patient without artifical immortalisation. Cells display a complete loss of TP53 expression and function despite having no loss of chromosome 17p and show highly similar pan-genomic similarity to the original patient tumor. Like the original tumor, the cells also expressed epithelial and mesenchymal characteristics. NUCOLL43 cells poses a very high degree of loss of heterozygosity which affected 85% of the genome.NUCOLL43 cells are resistant to cisplatin and rucaparib, but sensitive to paclitaxel, camptothecin and NVP-BEZ 235.
• Biosafety level: 1

Applications

• Application notes: NUCOLL43 was established from ascitic fluid from a female 57 year old white British patient without artifical immortalisation. Cells display a complete loss of TP53 expression and function despite having no loss of chromosome 17p and show highly similar pan-genomic similarity to the original patient tumor. Like the original tumor, the cells also expressed epithelial and mesenchymal characteristics. NUCOLL43 cells poses a very high degree of loss of heterozygosity which affected 85% of the...

Handling

• Format: Frozen
• Growth medium: RPMI-1640 media supplemented with 20% FBS and cultured at 37?‚°C in 5% CO2.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Franklin et al. 2018. Cancer Med. :. PMID: 30109783.
• Characterization and drug sensitivity of a novel human ovarian clear cell carcinoma cell line genomically and phenotypically similar to the original tumor.