Cat. #160394
Myok9 cell line
Cat. #: 160394
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Canine
Tissue: Skeletal
Disease: Muscle Disorders
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Nghiem
Institute: Texas A&M University
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Myok9 cell line
- Organism: Canine
- Tissue: Skeletal
- Disease: Muscle Disorders
- Model: Immortalised Line
- Conditional: No
- Description: This Myok9 cell line is derived from skeletal muscle from a genotypically normal dog and has been immortalized to study muscle disorders in canines. This cell line expresses dystrophin and other muscle-specific proteins during differentiation.
- Production details: Surgical biopsy of vastus lateralis muscle was performed on a 3-year old normal golden retriever. Myoblast extraction using pre-plate technique was performed. Primary cells were extracted and immunostained for desmin and PAX7. Immortalization was performed with lentivirus encoding SV40 large T antigen with puromycin resistance gene at MOI 2. Cells were selected with 1ug/ml puromycin for 3 days and passaged for 3 cycles.
- Biosafety level: 1
Target Details
- Target: canine muscle cell line
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- L?ÂÄÂpez et al. 2020. Mamm Genome. 31(3-4):95-109. PMID: 32246189.
