Cat. #162010
Melan-ep1 cell line
Cat. #: 162010
Organism: Mouse
Tissue: Skin
Disease: Hermansky-Pudlak syndrome
Model: Immortalised non-cancerous cell lines
£575.00
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Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Primary Citation: Suzuki T, et al. Nat Gene. 2002 Mar;30(3):321-4. PMID: 11836498
Tool Details
*FOR RESEARCH USE ONLY
- Name: Melan-ep1 cell line
- Organism: Mouse
- Tissue: Skin
- Disease: Hermansky-Pudlak syndrome
- Morphology: Small, oval-fusiform
- Model: Immortalised non-cancerous cell lines
- Description: The melan-ep1 and -ep2 cell lines are immortal melanocyte cell lines derived from neonatal Hps1ep/Hps1ep C57BL/6J mice. These cell lines lack ep (pale ear), a subunit of the biogenesis of lysosome-related organelles complex 3 (BLOC-3). BLOC-3 is involved in cargo recycling from melanosomes. Pathogenic variants in the human ortholog of ep, HPS1, cause a form of Hermansky-Pudlak syndrome (HPS), HPS type 1. HPS is a group of disorders characterised by hypopigmentation, bleeding tendency and other variable symptoms. These cells are a useful model for HPS and for the study of trafficking of cargoes towards lysosome-related organelles.
- Application: Melanocyte cell biology
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
- Storage conditions: liquid N2
