#162010

Melan-ep1 cell line

Cat. #162010

Melan-ep1 cell line

Cat. #: 162010

Organism: Mouse

Tissue: Skin

Disease: Hermansky-Pudlak syndrome

Model: Immortalised non-cancerous cell lines

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Elena Sviderskaya

Institute: St. George's University London

Primary Citation: Suzuki T, et al. Nat Gene. 2002 Mar;30(3):321-4. PMID: 11836498

Tool Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Melan-ep1 cell line
  • Organism: Mouse
  • Tissue: Skin
  • Disease: Hermansky-Pudlak syndrome
  • Morphology: Small, oval-fusiform
  • Model: Immortalised non-cancerous cell lines
  • Description: The melan-ep1 and -ep2 cell lines are immortal melanocyte cell lines derived from neonatal Hps1ep/Hps1ep C57BL/6J mice. These cell lines lack ep (pale ear), a subunit of the biogenesis of lysosome-related organelles complex 3 (BLOC-3). BLOC-3 is involved in cargo recycling from melanosomes. Pathogenic variants in the human ortholog of ep, HPS1, cause a form of Hermansky-Pudlak syndrome (HPS), HPS type 1. HPS is a group of disorders characterised by hypopigmentation, bleeding tendency and other variable symptoms. These cells are a useful model for HPS and for the study of trafficking of cargoes towards lysosome-related organelles.
  • Application: Melanocyte cell biology

Applications

  • Application: Melanocyte cell biology

Handling

  • Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
  • Storage conditions: liquid N2