Cat. #154151
MDA-MB-231 TetOn-3G ROCK2 cell line
Cat. #: 154151
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Heather Mckinnon ; Justin Bower ; Daniel Croft
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MDA-MB-231 TetOn-3G ROCK2 cell line
- Alternate name: Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2
- Cancer: Breast cancer
- Cancers detailed: Triple negative breast (TNBC)
- Research fields: Cancer;Cell biology;Drug development;Genetics
- Tool sub type: Continuous
- Parental cell: MDA-MB-231 D3H2LN-Luc cell line
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
- Model: Transgenic
- Conditional: Yes
- Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK2. Therefore ROCK2 expression is induced in the presence of doxycycline.
- Description: This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK2. ROCK2 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK2 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when impla...
- Biosafety level: 1
- Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
- Recommended controls: MDA-MB-231 D3H2LN-Luc parental line
Target Details
- Target: ROCK2
Handling
- Format: Frozen
- Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
- A novel small-molecule MRCK inhibitor blocks cancer cell invasion.
