MDA-MB-231 TetOn-3G ROCK2 cell line

The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging.

Contributors

Inventor Institute
Heather Mckinnon ; Justin Bower ; Daniel Croft Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 154151
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell biology;Drug development;Genetics
Organism: Human
Tissue: Breast
Model: Transgenic
Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
Cancer Types In Detail: Triple negative breast (TNBC)
Alternate name: Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2
Product description: This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK2. ROCK2 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK2 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when impla…
Conditional: Yes
Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK2. Therefore ROCK2 expression is induced in the presence of doxycycline.
Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
Parental cell line: MDA-MB-231 D3H2LN-Luc cell line
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
Recommended controls: MDA-MB-231 D3H2LN-Luc parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.

A novel small-molecule MRCK inhibitor blocks cancer cell invasion.

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