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#156525

MCF10A hRas_V12 cell line

Cat. #156525

MCF10A hRas_V12 cell line

Cat. #: 156525

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 8-10 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Cancer Model

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sarah-Maria Fendt

Institute: Vlaams Instituut voor Biotechnologie (VIB)

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: MCF10A hRas_V12 cell line
  • Alternate name: transforming protein p21, h-Ras, HRAS
  • Research fields: Cancer
  • Tool sub type: Continuous
  • Parental cell: MCF10A
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Cancer Model
  • Conditional: No
  • Description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity.
  • Biosafety level: 1
  • Additional notes: There is a negative control for this cell line which was made using the same pLA vector but not expressing the HRAS gene.

Target Details

  • Target: HRas

Applications

  • Application notes: There is a negative control for this cell line which was made using the same pLA vector but not expressing the HRAS gene.

Handling

  • Format: Frozen
  • Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)

References

  • Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.

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