Cat. #158349
JU77R cell line
Cat. #: 158349
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Lung
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sarah A Martin
Institute: Queen Mary University of London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: JU77R cell line
- Cancer: Lung cancer
- Cancers detailed: Lung
- Research fields: Cancer
- Tool sub type: Continuous
- Parental cell: JU77
- Organism: Human
- Tissue: Lung
- Disease: Cancer
- Growth properties: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20).
- Model: Cancer Model
- Production details: JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After approximately 2 months, colonies were picked and seeded in a 96-well plate with 10ug/ml ADI-PEG20. A surviving clone (JU777R) was gradually expanded and maintained in 1ug/ml ADI-PEG20.
- Cellosaurus id: TBC
Target Details
- Target: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20)
Handling
- Format: Frozen
- Growth medium: RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37?°C/5% CO2.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Locke et al. 2016. Cell Rep. 16(6):1604-1613. PMID: 27452468.
