#154112

Immortalised Human Fibroblast Cell Line

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Cat. #154112

Immortalised Human Fibroblast Cell Line

Cat. #: 154112

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Fibroblast

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Pranab K Das

Tool Details
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Immortalised Human Fibroblast Cell Line
  • Research fields: Cell biology
  • Parental cell: Human foreskin
  • Organism: Human
  • Tissue: Fibroblast
  • Growth properties: Cells were routinely passaged 1:4 at confluency
  • Model: Immortalised Line
  • Description: Immortalised human fibroblasts are widely employed as a model system for cell and molecular biology studies
  • Production details: Fibroblast cultures were establised from neonatal foreskin using standard methods. Fibroblasts were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.

Handling

  • Format: Frozen
  • Growth medium: DMEM:M199 (1:4) supplemented with 10% FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice