Cat. #153206
HCT 116 AXL KO Tet-inducible Cell Line
Cat. #: 153206
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sandra Van Schaeybroeck
Institute: Queen's University Belfast
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HCT 116 AXL KO Tet-inducible Cell Line
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Colorectal
- Research fields: Cancer;Cell biology;Drug development
- Tool sub type: Continuous
- Parental cell: HCT 116
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Growth properties: Invasion, migration
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Conditional knockdown of endogenous AXL expression upon treatment with Tetracycline
- Description: Receptor tyrosine kinase (RTK) screens identifed AXL as a protein which underpins the migratory/invasive phenotype in colorectal cancer (CRC) (Dunne et al., 2014). AXL was shown to be a poor prognostic marker and an important mediator of cell migration/invasiveness. The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.
- Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against AXL. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÄÂÄ?g/mL puromycin and induced with 2ÄÂÄ?g/ml doxycycline.
- Biosafety level: 1
- Recommended controls: HCT 116 parental line
- Cellosaurus id: CVCL_HG04
Target Details
- Target: AXL
Handling
- Format: Frozen
- Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
- AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.
