Cat. #154105
Halo nevus Melanocyte Cell Line
Cat. #: 154105
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Disease: Halo Nevus
Model: Primary line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Halo nevus Melanocyte Cell Line
- Alternate name: Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo
- Research fields: Cell biology;Immunology
- Parental cell: Halo nevus
- Organism: Human
- Disease: Halo Nevus
- Model: Primary line
- Conditional: No
- Description: Halo nevus is a mole that is surrounded by a depigmented ring or 'halo'. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research.
- Production details: Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Handling
- Format: Frozen
- Growth medium: HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
