Halo nevus Melanocyte Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Halo nevus Melanocyte Cell Line
• Alternate name: Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo
• Research fields: Cell biology;Immunology
• Parental cell: Halo nevus
• Organism: Human
• Disease: Halo Nevus
• Model: Primary line
• Conditional: No
• Description: Halo nevus is a mole that is surrounded by a depigmented ring or 'halo'. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research.
• Production details: Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10Â?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Â?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).

Handling

• Format: Frozen
• Growth medium: HAMs F10 supplemented with 10 ?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice