fR2 Cell Line

. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.

Contributors

Inventor Institute
Joyce Taylor-Papadimitriou ; Sidney Chang Cancer Research UK, Lincoln's Inn Fields Institute
Cat. #: 153246
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Immortalised Line
Growth properties: Adherent
Cancer Types In Detail: Breast cancer;Breast cancer
Primary citation: Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.
Product description: The fR2 Cell Line was established by infecting milk cultures with with simian vacuolating virus 40 (SV40). fR2 cells express Muc-1 (also known as HMFG-1 antigen), which is a mucin-like component of human milk fat globule membranes. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice. This cell line is positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5 cell line, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. This cell line could be useful for studying the relationship between transformation and differentiation in human mammary epithelial cells.
Gender: Female
CRISPR: Yes
Conditional: No
Production details: The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20
Cellosaurus ID: CVCL_2444
Disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37° C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10ug/ml insulin + 5ug/ml hydrocortisone + 10% FBS.
Mycoplasma free: Yes
References:

Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.

Citations And Reviews

Related Tools for Cell lines

Cat. # Tool Name
151425 TR146 Cell Line Key Info View Tool
151445 1-7HB2 Cell Line Key Info View Tool
151446 FLYRD18 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.