#153246

fR2 Cell Line

Cat. #153246

fR2 Cell Line

Cat. #: 153246

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Immortalised Line

£430.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Joyce Taylor-Papadimitriou ; Sidney Chang

Institute: Cancer Research UK, Lincoln's Inn Fields Institute

Primary Citation: Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: fR2 Cell Line
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Immortalised Line
  • Conditional: No
  • Description: The fR2 Cell Line was established by infecting milk cultures with with simian vacuolating virus 40 (SV40). fR2 cells express Muc-1 (also known as HMFG-1 antigen), which is a mucin-like component of human milk fat globule membranes.

    fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice.

    This cell line is positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, ...
  • Application: Investigating relationship between transformation and differentiation
  • Production details: The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
  • Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20
  • Cellosaurus id: CVCL_2444

Applications

  • Application: Investigating relationship between transformation and differentiation
  • Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37° C.

    Culture Medium: RPMI 1640 + 2mM Glutamine + 10ug/ml insulin + 5ug/ml hydrocortisone + 10% FBS.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.