Cat. #153291
FH fl/fl Cell Line
Cat. #: 153291
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Mouse
Tissue: Kidney
Disease: Cancer
Model: Conditional KO
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eyal Gottlieb
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: FH fl/fl Cell Line
- Alternate name: Fumarate hydratase, FH, fumarase, Fh1, Hereditary leiomyomatosis and renal cell cancer, HLRCC
- Research fields: Cancer;Drug development;Metabolism
- Organism: Mouse
- Tissue: Kidney
- Disease: Cancer
- Model: Conditional KO
- Conditional: Yes
- Conditional description: Deletion of fumarate hydratase can be achieved by intracellular expression of Cre recombinase
- Description: Fumarate hydratase which can be found in either a mitochondrial or cytosolic form - is an enzyme that catalyzes the reversible hydration and dehydration of fumarate to malate. Signal sequences located in the protein dictate the subcellular location of each FH isoenzyme. The cytosolic form is involved in the metabolism of amino acids and fumarate and the mitochondrial form is involved in the Krebs Cycle (tricarboxylic acid cycle or the citric acid cycle). Germline mutations of FH are responsible for hereditary leiomyomatosis and renal-cell cancer (HLRCC)1.
- Biosafety level: 1
- Cellosaurus id: CVCL_HG00
Target Details
- Target: Fumarate Hydratase
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: FH-/- CL 1 Cell Line ; FH-/- CL 19 Cell Line
References
- Frezza et al. 2011. Nature. 477(7363):225-8. PMID: 21849978.
- Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase.
- Pollard et al. 2007. Cancer Cell. 11(4):311-9. PMID: 17418408.
- Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway.
