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#161925

CAMA-1 Cell Line

Cat. #161925

CAMA-1 Cell Line

Cat. #: 161925

Availability: 8-10 weeks

Organism: Human

Tissue: Breast; Mammary gland

Model: Tumourigenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jorgen Fogh

Institute: Memorial Sloan-Kettering Cancer Center (MSK)

Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: CAMA-1 Cell Line
  • Cancer: Breast cancer
  • Organism: Human
  • Gender: Female
  • Tissue: Breast; Mammary gland
  • Donor: This cell line was established in 1975 from the pleural effusion of a 51-year-old Caucasian female with malignant adenocarcinoma of the breast.
  • Morphology: Epithelial
  • Growth properties: Adherent, patchy
  • Model: Tumourigenic
  • Model description: Tumourigenic in nude mice
  • Products or characteristics of interest: Karyotype: modal number = 80; range = 68 to 83. This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1
  • Description: CAMA-1 is a luminal-type human breast cancer cell line that displays rounded morphology in adherent tissue culture. These cells are considered Her2-negative and oestrogen-receptor/progesterone-receptor (ER/PR)-positive. They are responsive to oestrogen and sensitive to growth inhibition by tamoxifen. The CAMA-1 cells have an in-frame mutation in the E-cadherin gene, resulting in a truncated, non-functional protein. In addition, they have oncogenic mutations in PTEN and p53 and amplification of the cyclin D1 gene.
  • Application: 3D cell culture
  • Biosafety level: 1
  • Additional notes: The cells form poorly differentiated grade III tumours consistent with breast carcinoma.

Target Details

  • Target: Antigen expression: Blood type O; Rh +; HLA A10, A11, B12, B18

Applications

  • Application: 3D cell culture

Handling

  • Passage number: An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
  • Growth medium: EMEM supplemented with FBS to a final concentration of 10%
  • Temperature: 37° C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry Ice
  • Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
  • Storage conditions: Vapor phase of liquid nitrogen
  • Str profiling: Amelogenin: XCSF1PO: 10,12D13S317: 12D16S539: 11D5S818: 12,13D7S820: 8,11TH01: 8,9.3TPOX: 8vWA: 15D3S1358: 18D21S11: 32.2D18S51: 14,15Penta_E: 12,14Penta_D: 10,11D8S1179: 12,13FGA: 19,25D19S433: 13D2S1338: 17,21

References

  • Ji et al. 1994. Journal of Cellular Biochemistry. 54: 332-342. PMID: 8200913.
  • Van Horssen et al. 2012. Breast Cancer Research and Treatment. 136: 365-377. PMID: 23053649.

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