CAMA-1 Cell Line

CAMA-1 is a luminal-type human breast cancer cell line that displays rounded morphology in adherent tissue culture. These cells are considered Her2-negative and oestrogen-receptor/progesterone-receptor (ER/PR)-positive. They are responsive to oestrogen and sensitive to growth inhibition by tamoxifen. The CAMA-1 cells have an in-frame mutation in the E-cadherin gene, resulting in a truncated, non-functional protein. In […]

Contributors

Inventor Institute
Jorgen Fogh Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161925
Cancer types: Breast cancer
Organism: Human
Tissue: Breast; Mammary gland
Gender: Female
Model: Tumourigenic
Morphology: Epithelial
Growth properties: Adherent, patchy
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.
Product description: CAMA-1 is a luminal-type human breast cancer cell line that displays rounded morphology in adherent tissue culture. These cells are considered Her2-negative and oestrogen-receptor/progesterone-receptor (ER/PR)-positive. They are responsive to oestrogen and sensitive to growth inhibition by tamoxifen. The CAMA-1 cells have an in-frame mutation in the E-cadherin gene, resulting in a truncated, non-functional protein. In addition, they have oncogenic mutations in PTEN and p53 and amplification of the cyclin D1 gene.
Gender: Female
Additional notes: The cells form poorly differentiated grade III tumours consistent with breast carcinoma.
Model description: Tumourigenic in nude mice
Products or characteristics of interest: Karyotype: modal number = 80; range = 68 to 83. This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter–q21::?::6p11.2–6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Ji et al. 1994. Journal of Cellular Biochemistry. 54: 332-342. PMID: 8200913.

Van Horssen et al. 2012. Breast Cancer Research and Treatment. 136: 365-377. PMID: 23053649.

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