CAMA-1 Cell Line

CAMA-1 is a luminal-type human breast cancer cell line that displays rounded morphology in adherent tissue culture. These cells are considered Her2-negative and oestrogen-receptor/progesterone-receptor (ER/PR)-positive. They are responsive to oestrogen and sensitive to growth inhibition by tamoxifen. The CAMA-1 cells have an in-frame mutation in the E-cadherin gene, resulting in a truncated, non-functional protein. In […]

Contributors

Inventor Institute
Jorgen Fogh Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161925
Cancer types: Breast cancer
Organism: Human
Tissue: Breast; Mammary gland
Gender: Female
Model: Tumourigenic
Morphology: Epithelial
Growth properties: Adherent, patchy
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.
Product description: CAMA-1 is a luminal-type human breast cancer cell line that displays rounded morphology in adherent tissue culture. These cells are considered Her2-negative and oestrogen-receptor/progesterone-receptor (ER/PR)-positive. They are responsive to oestrogen and sensitive to growth inhibition by tamoxifen. The CAMA-1 cells have an in-frame mutation in the E-cadherin gene, resulting in a truncated, non-functional protein. In addition, they have oncogenic mutations in PTEN and p53 and amplification of the cyclin D1 gene.
Gender: Female
Additional notes: The cells form poorly differentiated grade III tumours consistent with breast carcinoma.
Model description: Tumourigenic in nude mice
Products or characteristics of interest: Karyotype: modal number = 80; range = 68 to 83. This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter–q21::?::6p11.2–6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Ji et al. 1994. Journal of Cellular Biochemistry. 54: 332-342. PMID: 8200913.

Van Horssen et al. 2012. Breast Cancer Research and Treatment. 136: 365-377. PMID: 23053649.

Related Tools for Cell lines

Cat. # Tool Name
151425 TR146 Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151503 1-7 CE1 Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.