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#162159

A549 LIMD1 WT Cas9 cell line

Cat. #162159

A549 LIMD1 WT Cas9 cell line

Cat. #: 162159

Availability: 8-10 weeks

Organism: Human

Tissue: Lung

Model: Genetically modified cell line; Knock out control

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Tyson Sharp

Institute: Queen Mary University of London

Primary Citation: Kathryn Davidson et. al. 2021. Cell Death Dis. 12(11): 1075. PMID: 34764236

Tool Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: A549 LIMD1 WT Cas9 cell line
  • Cancers detailed: Lung Cancer
  • Research fields: Cancer
  • Parental cell: A549 cell line
  • Organism: Human
  • Gender: Male
  • Tissue: Lung
  • Donor: White, 58-year-old male with lung cancer
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Genetically modified cell line; Knock out control
  • Crispr: Yes
  • Description: Cell line control for the A549 lung adenocarcinoma line knocked-out for the tumour suppressor gene LIMD1, which is commonly lost as an early event in lung oncogenesis. This gene encodes a scaffold protein suppressesing tumorigenesis via a number of different mechanisms. This line has been used to test drugs in LIMD1-deficient lung cancers, both in vitro an in vivo. When inoculated into NOD/SCID mice subcutaneously, CRISPR-Cas9-generated LUAD A549 LIMD1+/+ and LIMD1?/? cells generate LIMD1+/+ and LIMD1?/? tumours, respectively.

Handling

  • Format: Frozen
  • Growth medium: DMEM medium supplemented with 10% FCS and 1% penicillin/streptomycin solution
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Cultured in antibiotics: Yes
  • Mycoplasma free: Yes

Related Tools

  • Related tools: A549 LIMD1 KO Cas9 cell line

References

  • Kathryn Davidson et. al. 2021. Cell Death Dis. 12(11): 1075. PMID: 34764236

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