Anti-TIGAR [M2-P4H2]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-TIGAR [M2-P4H2]
• Research fields: Apoptosis and autophagy;Cancer;Genetics
• Clone: M2-P4H2
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: ELISA ; IHC ; IF ; WB
• Description: TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose- 2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose- 2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis.
• Immunogen: Synthetic peptide; IINFEEGREV (amino acids 239-248)
• Isotype: IgG1 kappa
• Myeloma used: P3X63Ag8.653
• Recommended controls: ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma

Target Details

• Target: TP53-induced glycolysis and apoptosis regulator (TIGAR)
• Tissue cell line specificity: ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma
• Target background: TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose-2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose-2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis.

Applications

• Application: ELISA ; IHC ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice