A mouse monoclonal antibody recognising the junction region of human PAX3-FOXO1 and PAX7-FOXO1 fusion proteins, used to study fusion-positive alveolar rhabdomyosarcoma.
| Institute |
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| National Human Genome Research Institute |
| SKU: | 160866 |
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| Product description: | Anti-PAX-FOXO1 [PaxF] is a mouse monoclonal antibody that recognises the junction region of the human PAX3-FOXO1 and PAX7-FOXO1 fusion proteins. It is used to detect and study PAX-FOXO1 fusion proteins, supporting research into fusion-positive alveolar rhabdomyosarcoma, fusion status and oncogenic mechanisms in rhabdomyosarcoma. Reported applications include chromatin immunoprecipitation, immunohistochemistry, immunofluorescence, immunoprecipitation, western blot and chromatin immunoprecipitation sequencing. |
| Alternate name: | PFM.2 |
| Additional notes: | Alveolar Rhabdomyosarcoma (ARMS) is an aggressive pediatric cancer and cases with fusion oncoproteins PAX7-FOXO1 and PAX3-FOXO1 have a poor prognosis. Determination of the fusion status is important for RMS diagnostics and therapies. This antibody is specific for the PAX3-FOXO1 and PAX7-FOXO1 and therefore is a valuable tool to study the oncogenesis of ARMS. |
| Isotype: | IgG2b kappa |
| Molecular weight: | 105 kDa |
| Immunogen: | LH conjugated with peptide PF corresponding to the PAX3-FOXO1 translocation region aa 100-117 (TIGNGLSPQNSIRHNLSL) in Freund’s adjuvant. |
| Myeloma used: | P3X63Ag8.653 |
| Cat. #: | 160866 |
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| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer |
| Application: | ChIP ; IHC ; IF ; IP ; WB ; ChIP-seq |
| Target: | The junction region of the PAX3-FOXO1 and the PAX7-FOXO1 fusion proteins |
| Reactivity: | Human |
| Clone: | PaxF |
| Host: | Mouse |
| Class: | Monoclonal |
| Concentration: | 1 mg/mL |
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| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Product stable at -20°C when stored undiluted. Store aliquoted, avoid repeated freeze-thaw cycles. |
| Shipping conditions: | Dry ice |
| References: |
Azorsa et al. 2021. Mod Pathol. 34(4):748-757. PMID: 33299109. Gryder et al. 2017. Cancer Discov. 7(8):884-899. PMID: 28446439. Cao et al. 2010. Cancer Res. 70(16):6497-508. PMID: 20663909. Khan et al. 1999. Proc Natl Acad Sci U S A. 96(23):13264-9. PMID: 10557309. |
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