Cat. #151194
Anti-p21 [AC8.3]
Cat. #: 151194
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: p21
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-p21 [AC8.3]
- Alternate name: Cyclin-Dependent Kinase Inhibitor 1A; CDK-Interacting Protein 1; CDKN1; CAP2; MDA-6; CIP1; SDI1; WAF1; P21; Melanoma Differentiation Associated Protein 6; Wild-Type P53-Activated Fragment 1; CDK-Interaction Protein 1; DNA Synthesis Inhibitor; P21CIP1; MDA6; PIC1
- Research fields: Cancer;Cell biology;Genetics
- Clone: AC8.3
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
- Description: AC8.3 can be used as a detection system for rapidly dividing cells and is recommended for use in tissue culture.
- Immunogen: His-tagged human p21 expressed in bacleva virus.
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: p21
- Target background: Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and overexpression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.
Applications
- Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Xu et al. 2010. Biol Pharm Bull. 33(5):743-51. PMID: 20460749.
- Targeting the Na(+)/K(+)-ATPase alpha1 subunit of hepatoma HepG2 cell line to induce apoptosis and cell cycle arresting.
- Petrides et al. 2008. J Comp Neurol. 507(5):1653-62. PMID: 18241050.
- Differential output of the high-sensitivity rod photoreceptor: AII amacrine pathway.
