Cat. #151727
Anti-MCM5 [A2.4B4.B7.A11.A8]
Cat. #: 151727
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Mini Chromosome Maintenance protein5 (MCM5)
Class: Monoclonal
Application: IF ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ron Laskey
Institute: University of Cambridge
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-MCM5 [A2.4B4.B7.A11.A8]
- Alternate name: Minichromosome Maintenance Complex Component 5; P1-CDC46; CDC46; Minichromosome Maintenance Deficient 5 (Cell Division Cycle 46); EC 3.6.4.12
- Research fields: Genetics
- Clone: A2.4B4.B7.A11.A8
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IF ; WB
- Description: MCM5 (Mini Chromosome Maintainance protein-5) is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. MCM5 is a member of the MCM family of chromatin-binding proteins, which have DNA dependent ATPase motifs in their central domain. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may have a role in cell cycle regulation. MCM proteins 2-7 form a family of DNA helicases implicated at the initiation step of DNA synthesis. It has been reported that immunocytochemical assessment of MCM5 expression may be used as a diagnostic tool for cervical cancer and urothelial neoplasia. MCM5 expression may also be applicable in the detection of a number of other cancers including bladder, lung and oesophageal.
- Immunogen: Bacterially expressed His-tagged human Mcm5 (amino acids 367-582)
- Isotype: IgG2b
- Myeloma used: Sp2/0-Ag14
- Recommended controls: HeLa, HL60
Target Details
- Target: Mini Chromosome Maintenance protein5 (MCM5)
- Tissue cell line specificity: HeLa, HL60
- Target background: MCM5 (Mini Chromosome Maintainance protein-5) is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. MCM5 is a member of the MCM family of chromatin-binding proteins, which have DNA dependent ATPase motifs in their central domain. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may have a role in cell cycle regulation. MCM proteins 2-7 form a family of DNA helicases implicated at the initiation step of DNA synthesis. It has been reported that immunocytochemical assessment of MCM5 expression may be used as a diagnostic tool for cervical cancer and urothelial neoplasia. MCM5 expression may also be applicable in the detection of a number of other cancers including bladder, lung and oesophageal.
Applications
- Application: IF ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -80° C
- Shipping conditions: Shipping at 4° C
References
- Stoeber et al. 2002. J Natl Cancer Inst. 94(14):1071-9. PMID: 12122098.
- Diagnosis of genito-urinary tract cancer by detection of minichromosome maintenance 5 protein in urine sediments.
