Cat. #161665
Anti-HLA-G [o1G]
Cat. #: 161665
Sub-type: Primary antibody
Availability: 3-4 weeks
Target: Membrane-bound form of HLA-G (full-length HLA-G1)
Class: Monoclonal
Application: Immunohistochemistry (Frozen), Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation. No western
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Daniel Geraghty
Institute: Fred Hutchinson Cancer Center
Primary Citation: lshitani et al.2003. Journal of lmmunology. 171(3):1376-84. PMID: 12874228
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-HLA-G [o1G]
- Cancer: Blood cancer;Breast cancer;Genitourinary cancer;Skin cancer
- Research fields: Developmental biology;Immunology
- Clone: 01G
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: The heavy chain is approximately 45 kDa
- Reactivity: Human
- Host: Mouse
- Application: Immunohistochemistry (Frozen), Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation. No western
- Description: HLA-G has long been assumed a major factor in immune accommodation because of its localized expression and low levels of allelic polymorphism. This MoAb were developed to examine the role of all three nonclassical class I Ags in the maternal immune response during pregnancy. 0lG was developed to react specifically with the membrane form of HLA-G.
- Isotype: IgG1
Target Details
- Target: Membrane-bound form of HLA-G (full-length HLA-G1)
- Molecular weight: The heavy chain is approximately 45 kDa
- Target background: HLA-G are expressed in the placenta, a membrane form and a soluble form, the latter containing an altered carboxyl terminu. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (?2 microglobulin). The heavy chain is anchored in the membrane.
Applications
- Application: Immunohistochemistry (Frozen), Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation. No western
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage buffer: PBS, pH 7.4
- Storage conditions: 4° C do not freeze
- Shipping conditions: Dry ice
References
- lshitani et al.2003.Journal of lmmunology. 171(3):1376-84.PMID: 12874228
- Lee et al.1995. Immunity.(5):591-600.PMID: 7584149
