Cat. #151157
Anti-ErbB3 [RTJ1]
Cat. #: 151157
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: ErbB3 (Her3)
Class: Monoclonal
Application: IHC ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Bill Gullick
Institute: Imperial Cancer Research Fund
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-ErbB3 [RTJ1]
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction
- Clone: RTJ1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 105 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IP ; WB
- Description: Monoclonal antibody directed against ErbB3 kinase, widely prevalent in GI and breast tumours.
- Immunogen: Synthetic Peptide from C terminus of human c-erbB3
- Immunogen uniprot id: P21860
- Isotype: IgM
- Myeloma used: NS0
Target Details
- Target: ErbB3 (Her3)
- Molecular weight: 105 kDa
- Target background: The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). c-erbB3 is normally found in non-dividing differentiated epithelial cells, neurones and hepatocytes. ErbB3 was found to be kinase impaired, having only 1/1000 the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as an allosteric activator. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2. It has approximately 80% prevalence in a wide variety of tumours of the gastro-intestinal tract, and approximately 20% prevalence in breast cancer. This antibody is specific against c-erbB-3 oncoprotein that is expressed in a variety of human normal, foetal and neoplastic epithelial tissues.
Applications
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Dry ice
References
- Li et al. 2017. Oncotarget. 8(40):67140-67151. PMID: 28978022.
- Lattanzio et al. 2016. Oncol Lett. 12(1):453-458. PMID: 27347164.
- Anagnostou et al. 2010. Cancer Epidemiol Biomarkers Prev. 19(4):982-91. PMID: 20332259.
- Analytic variability in immunohistochemistry biomarker studies.
- Kapitanovic et al. 2000. J Cancer Res Clin Oncol. 126(4):205-11. PMID: 10782893.
- Expression of erbB-3 protein in colorectal adenocarcinoma: correlation with poor survival.
- Naidu et al. 1998. Br J Cancer. 78(10):1385-90. PMID: 9823984.
- Expression of c-erbB3 protein in primary breast carcinomas.
- Srinivasan et al. 1998. J Pathol. 185(3):236-45. PMID: 9771476.
- Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types.
- Rajkumar et al. 1996. Clin Mol Pathol. 49(4):M199-202. PMID: 16696074.
- c-erbB3 protein expression in ovarian cancer.
- Rajkumar et al. 1993. J Pathol. 170(3):271-8. PMID: 8133400.
- Expression of the c-erbB-3 protein in gastrointestinal tract tumours determined by monoclonal antibody RTJ1.
