Cat. #151021
Anti-CyclinB1 [V152]
Cat. #: 151021
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Cyclin B1
Class: Monoclonal
Application: FACS ; IHC ; WB
Reactivity: Mammalian
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CyclinB1 [V152]
- Alternate name: CCNB1; Cyclin B1; G2/Mitotic-Specific Cyclin B1; CCNB
- Research fields: Cancer;Cell biology;Genetics
- Clone: V152
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Mammalian
- Host: Mouse
- Application: FACS ; IHC ; WB
- Description: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia.
- Immunogen: His tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HC1 and purified on Nickel beads.
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Cyclin B1
- Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia.
Applications
- Application: FACS ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: '-15° C to -25° C
- Shipping conditions: Dry ice
References
- Gurden et al. 2010. J Cell Sci. 123(Pt 3):321-30. PMID: 20053638.
- Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
- Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
- Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
- Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
- Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
- Brandeis et al. 1998. Proc Natl Acad Sci U S A. 95(8):4344-9. PMID: 9539739.
- Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
