Cat. #151037
Anti-Cdk1 [17 (A17)]
Cat. #: 151037
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Cyclin dependent kinase 1 (Cdk1, cdc2)
Class: Monoclonal
Application: ELISA ; IHC ; IP ; WB
Reactivity: Chicken ; Human ; Mouse ; Rat ; Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Cdk1 [17 (A17)]
- Alternate name: Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1
- Research fields: Cancer;Cell biology;Neurobiology
- Clone: 17 (A17)
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 34 kDa
- Strain: Balb/c
- Reactivity: Chicken ; Human ; Mouse ; Rat ; Xenopus laevis
- Host: Mouse
- Application: ELISA ; IHC ; IP ; WB
- Description: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
- Immunogen: A carboxy-terminal fragment starting at methionine 85 of p34 cdc2 (Xenopus laevis).
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Tonsil
Target Details
- Target: Cyclin dependent kinase 1 (Cdk1, cdc2)
- Molecular weight: 34 kDa
- Tissue cell line specificity: Tonsil
- Target background: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
Applications
- Application: ELISA ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.
- Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.
- Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.
- Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.
- Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.
- A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.
- Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.
- The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.
- Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.
- Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.
- Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.
- Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.