Cat. #151546
Anti-CDX1 [123a]
Cat. #: 151546
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-5 days
Target: CDX1
Class: Monoclonal
Application: ChIP ; IF ; WB ; ChIP-seq
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CDX1 [123a]
- Alternate name: Caudal Type Homeobox 1
- Research fields: Apoptosis and autophagy;Cancer;Genetics
- Clone: 123a
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 32 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ChIP ; IF ; WB ; ChIP-seq
- Description: CDX1 is an intestine-specific transcription factor with a role in directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. Cdx1 positively regulates its own expression in the small intestine and colon of fetus and adult and promotes cellular growth and differentiation in epithelial intestinal cells. A reduction in human Cdx1 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.
- Immunogen: Human CDX-1 N-terminal peptide
- Isotype: IgG1
- Myeloma used: P3/NS1/1-Ag4.1
- Recommended controls: HT55, LS174T, RCM1
Target Details
- Target: CDX1
- Molecular weight: 32 kDa
- Tissue cell line specificity: HT55, LS174T, RCM1
- Target background: CDX1 is an intestine-specific transcription factor with a role in directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. Cdx1 positively regulates its own expression in the small intestine and colon of fetus and adult and promotes cellular growth and differentiation in epithelial intestinal cells. A reduction in human Cdx1 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.
Applications
- Application: ChIP ; IF ; WB ; ChIP-seq
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Jones et al. 2015. Proc Natl Acad Sci U S A. 112(13):E1550-8. PMID: 25775580.
- The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation.
- Ashley et al. 2013. Cancer Res. 73(18):5798-809. PMID: 23867471.
- Stem cell differentiation and lumen formation in colorectal cancer cell lines and primary tumors.
- Yeung et al. 2011. Proc Natl Acad Sci U S A. 108(11):4382-7. PMID: 21368208.
- Hypoxia and lineage specification of cell line-derived colorectal cancer stem cells.
- Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. PMID: 20133591.
- Cancer stem cells from colorectal cancer-derived cell lines.
- Chan et al. 2009. Proc Natl Acad Sci U S A. 106(6):1936-41. PMID: 19188603.
- Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1.
