Anti-CD44 [P1G12]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD44 [P1G12]
• Research fields: Cell biology
• Clone: P1G12
• Tool sub type: Primary antibody
• Class: Monoclonal
• Molecular weight: 90-95kDa
• Strain: Hybridoma strain: Myeloma Strain NS-1/FOX-N
• Reactivity: Human, Macaques
• Host: Mouse
• Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
• Description: MoAb was developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. MoAb P1GI2 either increased cell adhesion to collagen or had no effect. It stimulated attachment of collagen to class III receptors and cell adhesion and shows a preferential association with cytoskeletal components.
• Immunogen: HT1080 human fibrosarcoma cell line
• Isotype: IgG1

Target Details

• Target: CD44(CRIII)
• Molecular weight: 90-95kDa
• Target background: CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Membrane glycoprotein termed class III( Anyibody P1G12) binds type I and VI collagen but not fibronectin.

Applications

• Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
• Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

• Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

References

• Wayner et al.1987. J. Cell Biol. 105(4):1873-84. PMID: 2822727