Cat. #161668
Anti-CD33
Cat. #: 161668
Sub-type: Primary antibody
Availability: 3-4 weeks
Target: CD33
Class: Monoclonal
Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Irwin Bernstein
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD33
- Cancer: Blood cancer
- Research fields: Cell biology
- Clone: P67.6
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: 39.82 kDa
- Reactivity: Human
- Host: Mouse
- Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
- Description: Anti-CD33, clone P676 is a mouse monoclonal antibody that detects CD33
- Immunogen: FMY9S5 cells expressing CD33
- Isotype: IgG1,?
Target Details
- Target: CD33
- Molecular weight: 39.82 kDa
- Target background: CD33 is expressed by acute myeloid leukemia (AML) cells in >80% of patients but not by normal hematopoietic stem cells, suggesting that elimination of CD33+ cells may be therapeutically beneficial.
Applications
- Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Shipping conditions: Dry ice
References
- Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178
