#161668

Anti-CD33

Cat. #161668

Anti-CD33

Cat. #: 161668

Sub-type: Primary antibody

Target: CD33

Class: Monoclonal

Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Irwin Bernstein

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178

Tool Details
Target Details
Applications
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CD33
  • Clone: P67.6
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Molecular weight: 39.82 kDa
  • Reactivity: Human
  • Host: Mouse
  • Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
  • Description: Anti-CD33, clone P676 is a mouse monoclonal antibody that detects CD33
  • Immunogen: FMY9S5 cells expressing CD33
  • Isotype: IgG1,?

Target Details

  • Target: CD33
  • Molecular weight: 39.82 kDa
  • Target background: CD33 is expressed by acute myeloid leukemia (AML) cells in >80% of patients but not by normal hematopoietic stem cells, suggesting that elimination of CD33+ cells may be therapeutically beneficial.

Applications

  • Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)

References

  • Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178