CRH trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia’s.
| Inventor | Institute |
|---|---|
| Peter Beverley | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151175 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology |
| Application: | FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB |
| Target: | CD3 |
| Reactivity: | Human |
| Clone: | UCH-T1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570. |
| Alternate name: | T3 complex |
|---|---|
| Product description: | CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia's. |
| Additional notes: | UCH-T1TM is a registered trademark of Cancer Research Technology, Limited. All rights reserved. |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 52 kDa |
| Immunogen: | Human infant thymocytes and Sezary cells. |
| Immunogen Uniprot ID: | P04234 |
| Myeloma used: | P3/NS1/1-Ag4.1 |
| Target background: | The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. UCH-T1 is considered a pan T-cell marker – it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia's. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Szary leukaemia's and approximately 70% of acute lymphoblastic leukaemia's of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions. |
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| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428. Oll Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192. Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991. rsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681. CD3-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation. Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291. Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome. Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645. Fn and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532. Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction. Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312. Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes. Salmern et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585. A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478. Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes. Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570. Distinctive Fn characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. |
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