Anti-CD3 monoclonal antibody [UCH-T1TM]

Cat. #151175

Anti-CD3 monoclonal antibody [UCH-T1TM]

Cat. #: 151175

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: CD3

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB

Reactivity: Human

Host: Mouse


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Peter Beverley

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Related Tools

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CD3 monoclonal antibody [UCH-T1TM]
  • Alternate name: T3 complex
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology
  • Clone: UCH-T1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 52 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB
  • Description: CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia's.
  • Immunogen: Human infant thymocytes and Sezary cells.
  • Immunogen uniprot id: P04234
  • Isotype: IgG1
  • Myeloma used: P3/NS1/1-Ag4.1
  • Additional notes: UCH-T1TM is a registered trademark of Cancer Research Technology, Limited. All rights reserved.

Target Details

  • Target: CD3
  • Molecular weight: 52 kDa
  • Target background: The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3d and 2X CD3), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia's. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Szary leukaemia's and approximately 70% of acute lymphoblastic leukaemia's of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.


  • Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB


  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4 C

Related Tools

  • Related tools: Anti-CD3, Recombinant [UCH-T1TM]


  • Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428.
  • Oll Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192.
  • Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991.
  • rsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.
  • CD3-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.
  • Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.
  • Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.
  • Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.
  • Fn and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.
  • Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
  • Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
  • Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.
  • Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.
  • Salmern et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.
  • A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
  • Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.
  • Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.
  • Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.
  • Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.
  • Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
  • Distinctive Fn characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.