Skip to main content

#161669

Anti-CD3

Cat. #161669

Anti-CD3

Cat. #: 161669

Sub-type: Primary antibody

Availability: 3-4 weeks

Target: CD3/T-cell receptor(TCR) complex: CD3?, one CD3?, one CD3?, one CD3?

Class: Monoclonal

Application: Flow cytometry

Reactivity: human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Claudio Anasetti

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Anasetti et al. 1990. Journal of Experimental Medicine. 172(6):1691-700. PMID: 2147949

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CD3
  • Cancer: Blood cancer
  • Research fields: Immunology
  • Clone: BC3
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Strain: Application Notes: For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 Âľg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
  • Reactivity: human
  • Host: Mouse
  • Application: Flow cytometry
  • Description: This anti-CD3 antibody can induce a sustained state of antigenspecific nonresponsiveness in unprimed human T cells. High concentration of soluble anti-CD3 antibody can condition T cells to become anergic in response to allogeneic MHC.
  • Immunogen: 2 x 10^6 PHA
  • Isotype: IgG2b

Target Details

  • Target: CD3/T-cell receptor(TCR) complex: CD3?, one CD3?, one CD3?, one CD3?
  • Target alternate names: T3,
  • Target background: CD3? is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3?, one CD3?, one CD3?, one CD3? (CD247), and a T-cell receptor (?/? or ?/?) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytesAntigen recognition by human T cells is mediated by the ?/? polymorphic chains of the TCR, noncovalently associated on the cell surface with a group of five invariant polypeptides which collectively represent the CD3 complex responsible for signal transduction . Binding of an antibody to TCR or to CD3 can block T cell responses not merely by steric hindrance of TCR but also by altering cellular function.

Applications

  • Application: Flow cytometry
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)

Handling

  • Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C. Please note the storage condition for this antibody has been changed from -20° C to between 2° C and 8° C.
  • Shipping conditions: Dry ice

References

  • Anasetti et al.1990.Journal of Experimental Medicine.172(6):1691-700.PMID: 2147949

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.