EmGFP-SUMO2 + mCherry HSF1 expressing U-2 OS cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: EmGFP-SUMO2 + mCherry HSF1 expressing U-2 OS cell line
• Parental cell: U-2 OS
• Clone: U2OS -1367+1401-B9
• Organism: Human
• Gender: Female
• Tissue: Bone
• Donor: 15 years old, white
• Disease: Cancer
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Transgenic
• Model description: Expression of the small ubiquitin-related modifier 2 (SUMO2) with an Emerald Green Fluorescent Protein (EmGFP) tag, as well as the fluorescently mCherry tagged Heat Shock Transcription Factor 1 (HSF1)
• Crispr: No
• Conditional: No
• Description: U-2 OS cell line derivative expressing the small ubiquitin-related modifier 2 (SUMO2) with an Emerald Green Fluorescent Protein (EmGFP) tag, as well as the fluorescently mCherry tagged Heat Shock Transcription Factor 1 (HSF1)
• Application: Cell culture, live cell imaging
• Biosafety level: BSL-2

Target Details

• Target: SUMO2, HSF1
• Target background: SUMO2 binds to proteins as part of the post-translational modification and is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. HSF1 is the main mediator of the stress response located in the cell nucleus, and is also involved in non-stress regulation, such as metabolism and development.

Applications

• Application: Cell culture, live cell imaging

Handling

• Format: Frozen
• Atmosphere: humidified CO2 atmosphere
• Storage conditions: Liquid Nitrogen
• Cultured in antibiotics: Yes, penicillin/streptomycin

References

• Holcakova et al. (2017). Breast Cancer Res Treat. 163(3):475-484, PMID: 28349272
• Nekulova et al. (2016). BMC Cancer, 10
• 16(1):782, PMID: 27724925