Cat. #161685
Spag17/Tomato-EGFP/CMV-Cre mouse
Cat. #: 161685
Disease: Development
Model: Knock-out
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Contributor
Inventor: Maria E Teves Ph.D.
Institute: Virginia Commonwealth University
Primary Citation: Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Spag17/Tomato-EGFP/CMV-Cre mouse
- Research fields: Cell biology;Organ fibrosis;Primary cilia dyskinesia;Skeletal dysplasia
- Disease: Development
- Model: Knock-out
- Crispr: No
- Description: This is a knockout mouse model for the global deletion of the Spag17 gene linked to a Tomato-EGFP system for cell linage tracing. This mouse model has the potential to generate a knockout for Spag17 when crossed with a Cre mouse. Cells will express an EGFP fluorophore for in vivo tracking if they are deficient in Spag17 and presents an avanue for studying primary cili dyskinesia, development, skeletal dysplasia, primary cilia disfunction, aging, infertility, and multi-organ fibrosis
- Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
- Genetic background: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
- Phenotype: Multi-organ fibrosis
- Zygosity: Homozygous
- Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
- Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice dem...
Application Details
- Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
References
- Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344
- Teves et al. PLoS One 10(5) PMID: 26017218
