#161682

Spag17 flox mouse

Cat. #161682

Spag17 flox mouse

Cat. #: 161682

Disease: Development

Model: Knock-out

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Contributor

Inventor: Maria E Teves Ph.D.

Institute: Virginia Commonwealth University

Primary Citation: Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344

Tool Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: Spag17 flox mouse
  • Research fields: Cell biology;Organ fibrosis;Primary cilia dyskinesia;Skeletal dysplasia
  • Disease: Development
  • Model: Knock-out
  • Crispr: No
  • Description: Spag17 flox mouse to generate the Spag17 knockout mouse after Cre recombination. Spag17 is a complex gene associated with cilia and microtubule cytoskeleton. Loss of Spag17 leads to developmental defects, primary cilia dyskinesia, accelerated aging, male infertility, and multi-organ fibrosis. The novel flox mouse model that was created has the potential to generate a knockout for Spag17 once crossed with a Cre mouse.
  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
  • Genetic background: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
  • Phenotype: Multi-organ fibrosis
  • Zygosity: Homozygous
  • Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
  • Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice dem...

Application Details

  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging

References

  • Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344