Spag17 flox mouse

Tool Details

*FOR RESEARCH USE ONLY

• Tool name: Spag17 flox mouse
• Disease: Development; Fibroblast Biology and Aging; Male infertility; Fibrosis
• Model: Knock-out
• Crispr: No
• Description: Spag17 flox mouse to generate the Spag17 knockout mouse after Cre recombination. Spag17 is a complex gene associated with cilia and microtubule cytoskeleton. Loss of Spag17 leads to developmental defects, primary cilia dyskinesia, accelerated aging, male infertility, and multi-organ fibrosis. The novel flox mouse model that was created has the potential to generate a knockout for Spag17 once crossed with a Cre mouse.
• Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
• Genetic background: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
• Phenotype: Multi-organ fibrosis
• Zygosity: Homozygous
• Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
• Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice dem...

Application Details

• Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging

References

• Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344