Cat. #154182
Siglec-F KO mouse
Cat. #: 154182
Sub-type: Mouse
Availability: 8-10 weeks
Model: Knock-Out
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Paul Crocker
Institute: University of Dundee
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Siglec-F KO mouse
- Alternate name: Siglec-8, Sialic Acid Binding Ig Like Lectin 8, SAF-2,
- Research fields: Cell biology;Immunology
- Tool sub type: Mouse
- Model: Knock-Out
- Conditional: No
- Description: Siglecs-8 and -F are paralogous membrane proteins highly expressed on human and mouse eosinophils respectively. They are members of the CD33-related sialic acid binding Ig-like lectin family and contain immunoreceptor tyrosine based inhibitory motifs (ITIM) and ITIM-like motifs in their cytoplasmic tails that are implicated in negative regulatory functions
- Genetic background: Siglec FR114D knock in mice, carrying an Arg114 to Asp mutation in the Siglec F gene predicted to abolish sialic acid binding were generated by Taconic Artemis using C57Bl/6 ES cells (targeting strategy illustrated in PMID: 24698729). Further crossing of homozygous Siglec FR114D mice with Tg (Nes-Cre)1Wme/J(Bal1-Cre) mice produced offspring with mosaicism/partial deletion of exons 6-9 in adult organs (including germline) resulting in the truncated Siglec F knock out allele. Mosaic offspring were then used to generate Siglec F KO mice
- Zygosity: Homozygous
- Strain: C57BL/6
- Production details: Siglec FR114D knock in mice, carrying an Arg114 to Asp mutation in the Siglec F gene predicted to abolish sialic acid binding were generated by Taconic Artemis using C57Bl/6 ES cells (targeting strategy illustrated in PMID: 24698729). Further crossing of homozygous Siglec FR114D mice with Tg (Nes-Cre)1Wme/J(Bal1-Cre) mice produced offspring with mosaicism/partial deletion of exons 6-9 in adult organs (including germline) resulting in the truncated Siglec F knock out allele. Mosaic offspring were then used to generate Siglec F KO mice
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Siglec-F
References
- McMillan et al. 2014. Immunol Lett. 160(1):11-16. PMID: 24698729.
- Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.
