SAR-22-097F mouse line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: SAR-22-097F mouse line
• Cancer: Carcinoma
• Research fields: Cancer
• Disease: Non-alcoholic fatty liver disease (NAFLD)
• Model: Knock-In
• Crispr: Yes
• Conditional: No
• Description: Mouse line with cysteine 75 of AEG-1 mutated to serine. AEG-1-C75S mice might be more prone to developing NASH and HCC which could help design new therapeutic strategies. Findings identify a novel role of AEG-1 palmitoylation regulating NASH and HCC thereby establishing the rationale for using inhibitors of AEG-1 depalmitoylating enzymes for the treatment of NASH and HCC.
• Application: RNA-Seq, Western Blot, and Immunofluorescence
• Genetic background: AEG-1-C75S mice were created in C57BL/6/J background. ATG-1-C75S heterozygotexheterozygote mating was performed to generate AEG-1-WT adn AEG-1-C75S littermates
• Phenotype: Unaffected litter size of AEG-1-C75S mice compared to WT adn overall showing milder and different phenotype compared to AEG-1-/- mice
• Strain: C57BL/6/J
• Production details: Knock-in mice by CRISPR/Cas9 in which Cys75 of AEG-1 was mutated to Ser (AEG-1-C75S). AEG-1-C75S mice were created in C57BL/6/J background. ATG-1-C75S heterozygotexheterozygote mating was performed to generate AEG-1-WT adn AEG-1-C75S littermates
• Recommended controls: AEG-1 WT

Application Details

• Application: RNA-Seq, Western Blot, and Immunofluorescence

References

• Komaniecki et al. Biochemistry 62(2):543-553. PMID: 36548985