#161681

SAR-22-097F mouse line

Cat. #161681

SAR-22-097F mouse line

Cat. #: 161681

Disease: Non-alcoholic fatty liver disease (NAFLD)

Model: Knock-In

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Contributor

Inventor: Devanand Sarkar

Institute: Virginia Commonwealth University

Primary Citation: Komaniecki et al. Biochemistry 62(2):543-553. PMID: 36548985

Tool Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: SAR-22-097F mouse line
  • Cancer: Carcinoma
  • Research fields: Cancer
  • Disease: Non-alcoholic fatty liver disease (NAFLD)
  • Model: Knock-In
  • Crispr: Yes
  • Conditional: No
  • Description: Mouse line with cysteine 75 of AEG-1 mutated to serine. AEG-1-C75S mice might be more prone to developing NASH and HCC which could help design new therapeutic strategies. Findings identify a novel role of AEG-1 palmitoylation regulating NASH and HCC thereby establishing the rationale for using inhibitors of AEG-1 depalmitoylating enzymes for the treatment of NASH and HCC.
  • Application: RNA-Seq, Western Blot, and Immunofluorescence
  • Genetic background: AEG-1-C75S mice were created in C57BL/6/J background. ATG-1-C75S heterozygotexheterozygote mating was performed to generate AEG-1-WT adn AEG-1-C75S littermates
  • Phenotype: Unaffected litter size of AEG-1-C75S mice compared to WT adn overall showing milder and different phenotype compared to AEG-1-/- mice
  • Strain: C57BL/6/J
  • Production details: Knock-in mice by CRISPR/Cas9 in which Cys75 of AEG-1 was mutated to Ser (AEG-1-C75S). AEG-1-C75S mice were created in C57BL/6/J background. ATG-1-C75S heterozygotexheterozygote mating was performed to generate AEG-1-WT adn AEG-1-C75S littermates
  • Recommended controls: AEG-1 WT

Application Details

  • Application: RNA-Seq, Western Blot, and Immunofluorescence

References

  • Komaniecki et al. Biochemistry 62(2):543-553. PMID: 36548985