Raf KI D486A Mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: Raf KI D486A Mouse
• Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
• Tool sub type: Mouse
• Disease: Cancer
• Conditional: No
• Description: Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
• Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
• Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: Raf1 D486A (oncogenic mutant)

References

• Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
• BRAF inactivation drives aneuploidy by deregulating CRAF.
• Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
• CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.