#151475

Raf KI D486A Mouse

Cat. #151475

Raf KI D486A Mouse

Cat. #: 151475

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: Raf KI D486A Mouse
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Conditional: No
  • Description: Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
  • Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
  • Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Raf1 D486A (oncogenic mutant)

References

  • Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
  • BRAF inactivation drives aneuploidy by deregulating CRAF.
  • Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
  • CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.