Raf-1FF/FF knockin mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: Raf-1FF/FF knockin mouse
• Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
• Tool sub type: Mouse
• Disease: Cancer
• Model: Knock-In
• Description: A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
• Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
• Strain: C57BL/6
• Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
• Additional notes: Genetic Background / Cross History : C57BL/6

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: Raf1 Y340F Y341F (inactive mutant)

Related Tools

• Related tools: MEF Raf1 FF/FF KI Cell Line

References

• Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
• MEK kinase activity is not necessary for Raf-1 function.