#151477

Raf-1FF/FF knockin mouse

Cat. #151477

Raf-1FF/FF knockin mouse

Cat. #: 151477

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

Model: Knock-In

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: Raf-1FF/FF knockin mouse
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Model: Knock-In
  • Description: A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
  • Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
  • Strain: C57BL/6
  • Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
  • Additional notes: Genetic Background / Cross History : C57BL/6

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Raf1 Y340F Y341F (inactive mutant)

Related Tools

  • Related tools: MEF Raf1 FF/FF KI Cell Line

References

  • Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  • MEK kinase activity is not necessary for Raf-1 function.