#151534

Pdgfrb-Cre Mouse

Cat. #151534

Pdgfrb-Cre Mouse

Cat. #: 151534

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

Model: Conditional KO

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ralf H. Adams

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: Pdgfrb-Cre Mouse
  • Research fields: Cancer;Genetics;Immunology;Tissue-specific biology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: Pdgfrb-Cre enables conditional, pericyte and smooth vascular muscle cell knockout/knockin/transgene expression of loxP flanked genes.
  • Description: Cre expressed under Pdgfrb promoter enabling conditional recombine expression in pericytes and smooth vascular muscle cells (tissue-specific loxP knockout/knockin)
  • Genetic background: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.
  • Production details: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Cre recombinase under Pdgfrb promoter

References

  • Kogata et al. 2009. Genes Dev. 23(19):2278-83. PMID: 19797768.
  • Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction.
  • Foo et al. 2006. Cell. 124(1):161-73. PMID: 16413489.
  • Ephrin-B2 controls cell motility and adhesion during blood-vessel-wall assembly.