Cat. #151534
Pdgfrb-Cre Mouse
Cat. #: 151534
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Cancer
Model: Conditional KO
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ralf H. Adams
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Pdgfrb-Cre Mouse
- Research fields: Cancer;Genetics;Immunology;Tissue-specific biology
- Tool sub type: Mouse
- Disease: Cancer
- Model: Conditional KO
- Conditional: Yes
- Conditional description: Pdgfrb-Cre enables conditional, pericyte and smooth vascular muscle cell knockout/knockin/transgene expression of loxP flanked genes.
- Description: Cre expressed under Pdgfrb promoter enabling conditional recombine expression in pericytes and smooth vascular muscle cells (tissue-specific loxP knockout/knockin)
- Genetic background: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.
- Production details: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Cre recombinase under Pdgfrb promoter
References
- Kogata et al. 2009. Genes Dev. 23(19):2278-83. PMID: 19797768.
- Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction.
- Foo et al. 2006. Cell. 124(1):161-73. PMID: 16413489.
- Ephrin-B2 controls cell motility and adhesion during blood-vessel-wall assembly.
