#153874

Oct1/2 Knock Out Mouse

Cat. #153874

Oct1/2 Knock Out Mouse

Cat. #: 153874

Sub-type: Mouse

Availability: 6-8 weeks

Model: Knock-Out

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Alfred Schinkel

Institute: Netherlands Cancer Institute

Tool Details
Handling
Target Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: Oct1/2 Knock Out Mouse
  • Alternate name: SLC22A1/2, Solute Carrier Family 22 Member 1/2, Organic Cation Transporter 1/2
  • Research fields: Cancer;Cell signaling and signal transduction;Drug development
  • Tool sub type: Mouse
  • Model: Knock-Out
  • Conditional: No
  • Description: Oct1/2 are polyspecific organic cation transporters in the liver, kidney, intestine, and other organs. They are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.
  • Application: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.
  • Genetic background: The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations
  • Zygosity: Homozygous
  • Production details: The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations
  • Breeding information: The colony is maintained by mating doubly homozygous mic
  • Additional notes: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations. The Oct1/2 mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Oct1 and Oct2

Application Details

  • Application: Useful for studies of drug transport in the liver, intestine and kidneys. OCT1 and OCT2 determine renal secretion of small organic cations.

References

  • Jonker et al. 2003. Mol Cell Biol. 23(21):7902-8. PMID: 14560032.
  • Deficiency in the organic cation transporters 1 and 2 (Oct1/Oct2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations.
  • Jonker et al. 2001. Mol Cell Biol. 21(16):5471-7. PMID: 11463829.
  • Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene.