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#151552

LFA1 -/- Mouse

Cat. #151552

LFA1 -/- Mouse

Cat. #: 151552

Sub-type: Mouse

Availability: 8-10 weeks

Disease: Inflammatory disease

Model: Knock-Out

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Contributor

Inventor: Nancy Hogg

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: LFA1 -/- Mouse
  • Alternate name: LFA-1, Integrin beta-2, Complement receptor C3 subunit beta, CD18
  • Research fields: Genetics;Immunology
  • Tool sub type: Mouse
  • Disease: Inflammatory disease
  • Model: Knock-Out
  • Conditional: No
  • Description: In vivo study of LFA-1 knockout; in vivo study of inflammation and leukocyte recruitment;
  • Genetic background: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.
  • Strain: C57BL/6
  • Production details: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Lymphocyte Function-associated Antigen-1 (LFA1)

References

  • Henderson et al. 2001. J Exp Med. 194(2):219-26. PMID: 11457896.
  • The use of lymphocyte function-associated antigen (LFA)-1-deficient mice to determine the role of LFA-1, Mac-1, and alpha4 integrin in the inflammatory response of neutrophils.
  • Berlin-Rufenach et al. 1999. J Exp Med. 189(9):1467-78. PMID: 10224287.
  • Lymphocyte migration in lymphocyte function-associated antigen (LFA)-1-deficient mice.

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