Cat. #151550
Integrin a6b4 Mouse
Cat. #: 151550
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Cancer
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Fiona Watt
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Integrin a6b4 Mouse
- Cancer: Skin cancer
- Cancers detailed: Squamous cell carcinoma
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Genetics;Stem cell biology
- Tool sub type: Mouse
- Disease: Cancer
- Conditional: Yes
- Conditional description: Conditional expression of integrins under involucrin promoter giving tissue-specific suprabasal epidermal expression.
- Description: Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin. Ninety six percent of alpha6beta4 transgenic mice develop squamous cell carcinoma, compared to 44% wildtype. Of those mice developing tumours, 68% alpha6beta4 develop metastasis, compared to 8% wildtype. TGF-beta signalling is disrupted in alpha6beta4 transgenic mice.
- Genetic background: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
- Phenotype: Mice present phenotype very similar to human psoriasis disease.
- Zygosity: Heterozygous
- Production details: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Alpha6Beta4 Integrin
References
- Owens DM et al, Journal of Cell Science, 2003, v116 pp3783-3791PMID: 12902406
