FGFR2III Floxed Mouse

Conditional knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout

Contributors

Inventor Institute
Clive Dickson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151654
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)
disease: Cancer
Model: Conditional KO
Zygosity: Homozygous
Strain: C57BL/6
Product description: Conditional knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout
Conditional: Yes
Conditional description: LoxP flanked IIIb exon of FGFR2 gene enabling tissue-specific cre-mediated knockout of IIIb isoform.
Genetic background: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.
Production details: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.

The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.

De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.

An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.

Related Tools for Mouse models

Cat. # Tool Name
151477 Raf-1FF/FF knockin mouse Key Info View Tool
151481 DLL1 Floxed Mouse Key Info View Tool
151550 Integrin a6b4 Mouse Key Info View Tool
151554 SAP1 Mouse Key Info View Tool
151555 MUC1 Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.