#151654

FGFR2III Floxed Mouse

Cat. #151654

FGFR2III Floxed Mouse

Cat. #: 151654

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

Model: Conditional KO

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Contributor

Inventor: Clive Dickson

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: FGFR2III Floxed Mouse
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Gastric
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: LoxP flanked IIIb exon of FGFR2 gene enabling tissue-specific cre-mediated knockout of IIIb isoform.
  • Description: Conditional knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout
  • Genetic background: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Production details: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)

Related Tools

  • Related tools: FGFR2b Mouse

References

  • Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
  • The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
  • De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
  • An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.