Cat. #151654
FGFR2III Floxed Mouse
Cat. #: 151654
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer
Model: Conditional KO
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Contributor
Inventor: Clive Dickson
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: FGFR2III Floxed Mouse
- Tool sub type: Mouse
- Disease: Cancer
- Model: Conditional KO
- Conditional: Yes
- Conditional description: LoxP flanked IIIb exon of FGFR2 gene enabling tissue-specific cre-mediated knockout of IIIb isoform.
- Description: Conditional knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout
- Genetic background: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.
- Zygosity: Homozygous
- Strain: C57BL/6
- Production details: An Fgfr2 targeting vector, containing a loxP flanked IIIb exon and a loxP flanked resistance cassette, was transfected into 129Ola ES cells.Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice to generate heterozygotes. Heterozygotes were bred to homozygosity to generate Fgfr2bflox/flox mice.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)
Related Tools
- Related tools: FGFR2b Mouse
References
- Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
- The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
- De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
- An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.
