Bmx Cre ERT2 Mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: Bmx Cre ERT2 Mouse
• Research fields: Developmental biology;Genetics;Immunology;Neurobiology;Tissue-specific biology
• Tool sub type: Mouse
• Disease: Cancer
• Model: Conditional KO
• Conditional: Yes
• Conditional description: Conditional Cre-ERT2 expression under Bmx promoter enabling tissue-specific recombinase in arterial endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen) enabling inducible translocation of Cre-ERT2 to nucleus.
• Description: The estragen receptor (ERT2) under the Bone marrow x (Bmx) promoter (Bmx-Cre-ERT2) mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in arterial endothelial cells. The Bmx-Cre-ERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP flanked genes in endothelial cells.Non-induced Bmx-Cre-ERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Bmx-Cre-ERT2 mice demonstrate high penetrance in endothelial cells (95%+), significantly higher than existing endothelial Cre models currently available.
• Genetic background: A Bmx-Cre-ERT2 transgene vector, containing a genomic VECad promoter fragment fused to a Cre-ERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Bmx-Cre-ERT2 transgene.
• Zygosity: Heterozygous
• Production details: A Bmx-Cre-ERT2 transgene vector, containing a genomic VECad promoter fragment fused to a Cre-ERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Bmx-Cre-ERT2 transgene.

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: BMX, CreERT2

References

• Murphy et al. 2014. Proc Natl Acad Sci U S A. 111(50):18007-12. PMID: 25468970.
• Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels.
• Noels et al. 2014. Arterioscler Thromb Vasc Biol. 34(6):1209-20. PMID: 24723559.
• Schober et al. 2014. Nat Med. 20(4):368-76. PMID: 24584117.
• Deficiency of endothelial CXCR4 reduces reendothelialization and enhances neointimal hyperplasia after vascular injury in atherosclerosis-prone mice.
• MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1.
• Ehling et al. 2013. Development. 140(14):3051-61. PMID: 23785053.
• Notch controls retinal blood vessel maturation and quiescence.