#154602

ZR-75-1 [XIV-17] cell line

Cat. #154602

ZR-75-1 [XIV-17] cell line

Cat. #: 154602

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Cancer Model

£430.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Lambert Dorssers

Institute: Erasmus University Medical Center (Erasmus MC)

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: ZR-75-1 [XIV-17] cell line
  • Tool sub type: Continuous
  • Parental cell: ZR-75-1
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Cancer Model
  • Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in advanced disease cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Retroviral insertion mutagenesis was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method the main genes conferring estrogen independence in human breast cancer cells where identified. Genes located in the ...
  • Production details: ZR-75-1 cells were infected with amphotropic, defective murine retrovirus and plated in medium containing 1uM of 4-hydroxy-tamoxifen. Within 5 weeks after the start of selection proliferating colonies were individually picked and expanded to stable cell lines
  • Biosafety level: 1

Target Details

  • Target: Breast cancer anti-estrogen resistance genes

Applications

  • Application notes: Cell line with unique integration events in the following genes, of which one is the most likely cause for estrogen independence: KPNA3 and AP2S1

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS)
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.