![ZR-75-1 EGFR [ZR-HERc 1F)] cell line](https://cancertools.org/wp-content/uploads/161197.jpg)
Cat. #154629
ZR-75-1 EGFR [ZR-HERc 1F)] cell line
Cat. #: 154629
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lambert Dorssers
Institute: Erasmus University Medical Center (Erasmus MC)
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: ZR-75-1 EGFR [ZR-HERc 1F)] cell line
- Alternate name: Epidermal Growth Factor Receptor; Erb-B2 Receptor Tyrosine Kinase 1; ERBB1; HER1
- Cancer: Gynaecologic cancer
- Cancers detailed: Mammary ductal carcinoma
- Research fields: Cancer;Drug development
- Tool sub type: Continuous
- Parental cell: ZR-75-1
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Cancer Model
- Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype.Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the parental (Cat No 154547).This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
- Production details: Cells were established by infection of ZR-75-1 cells with cell free supernatant of NT/HERc virus producing psi-2 cells for 17h in the presence of 8uM polybrene. Clones were selected in media containing 1mg/ml G418 and expanded in estradiol containing media.
- Biosafety level: 1
Target Details
- Target: EGFR
Applications
- Application notes: The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- van Agthoven et al. 1992. Cancer Res. 52(18):5082-8. PMID: 1516065.
