Cat. #153170
T47D-STn Cell Line
Cat. #: 153170
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joy Burchell ; Joyce Taylor-Papadimitriou
Institute: King's College London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: T47D-STn Cell Line
- Cancer: Breast cancer
- Cancers detailed: Breast cancer
- Research fields: Cancer;Drug development
- Tool sub type: Continuous
- Parental cell: T47D
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Conditional: No
- Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen.T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells.Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
- Biosafety level: 1
Target Details
- Target: N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 - ST6GalNAc-1
Handling
- Format: Frozen
- Growth medium: DMEM 10% FCS, 500 ?g/ml G418, 300 ?g/ml hygromycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: T47D-E2J Cell Line
References
- Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
- The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
