T47D-STn Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: T47D-STn Cell Line
• Cancer: Breast cancer
• Cancers detailed: Breast cancer
• Research fields: Cancer;Drug development
• Tool sub type: Continuous
• Parental cell: T47D
• Organism: Human
• Tissue: Breast
• Disease: Cancer
• Conditional: No
• Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen.T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells.Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
• Biosafety level: 1

Target Details

• Target: N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 - ST6GalNAc-1

Handling

• Format: Frozen
• Growth medium: DMEM 10% FCS, 500 ?g/ml G418, 300 ?g/ml hygromycin
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: T47D-E2J Cell Line

References

• Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
• The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.